The mosquito-borne disease surveillance program was established in 1977 to monitor and detect arbovirus activities in an effort to minimize the risk of human infections throughout Lee County. The program consists of four sections, which include sentinel chicken surveillance, mosquito population monitoring, laboratory testing, and human case investigation. There are 17 sentinel chicken sites located throughout the county to access local mosquito transmission of West Nile virus (WNV), St. Louis Encephalitis (SLEV), and Eastern Equine Encephalitis (EEEV) in birds. Mosquito population monitoring utilizes specialized adult mosquito trapping techniques such as Center for Disease Control (CDC) light traps to monitor levels of the vector mosquito Culex nigripalpus, and BG traps to monitor Aedes aegypti and Aedes albopictus. Our laboratory testing capabilities include the analysis of sentinel chicken sera samples for WNV, SLEV, and EEEV viral antibody response through ELISA testing; and the analysis of vector mosquitoes for WNV, SLEV, EEEV, DENGUE, Chikungunya (CHIKV), Zika (ZIKV), and Yellow Fever through RT-PCR for the presence of viral RNA. This data indicates the presence of infected or potentially infected mosquitoes in our local area. Human case investigation involves determining when and where a disease was contracted. A close working relationship with our local Florida Department of Health helps to determine when and where the mosquito-borne disease was contracted and whether it was acquired locally or travel related outside of Lee County or outside the country.